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KMID : 0358219950220020163
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Korean Journal of Fertility and Sterility
1995 Volume.22 No. 2 p.163 ~ p.170
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Effect of Epidermal Growth Factor(EGF) on Early Embryonic Development in Mouse
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벼녀혜경
이호준/김성례/김해권/김문규
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Abstract
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Growth factors (GFs) produced by the embryo or by the maternal reproductive tract have been reported to regulate the embryonic development and differentiation. Among GFs, EGF as a mitogen plays a role in mitosis and functional differentiation of
trophectoderm cells in mouse. The present study was carried out to investigate the effect of EGF on development of mouse embryos and to localize EGF in the mouse oocytes and embryos, which has been reported to be detected in the reproductive
tract
in
mammals.
To investigate the effect of EGF on the development of the embryo, mouse 2-cell embryos were cultured to blastocysts stage in Ham's F10 medium, treated with EGF (10-50 ng/ml) for 72 hrs.
Immunocytochemistry was performed from oocyte to blastocyst stage with anti-EGF and anti-Mouse IgG, in order to determine the stage which EGF would e expressed in mouse.
Exogenous EGF (more than 10 ng/ml) in the culture medium improved the developmental and hatching rates in the mouse embryos. As a result of immunocytochemistry, the embryonic EGF was expressed after the late 4-cell stage. EGF is thought to
enhance
preimplantation ombryonic development and hatching.
Exogenous EGF in the culture medium is thought to activate EGF receptor in the late 4-cell embryos and to enhance blastulation and hatching in mouse embryos. It is concluded that EGF enhances the developmental ad hatching rates in the mouse
embryos.
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